Journal: Fluids and Barriers of the CNS
Article Title: Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis
doi: 10.1186/s12987-024-00609-6
Figure Lengend Snippet: Isolation of microvessels from frozen human brain. ( A ) Schematic representation of the method used to isolate brain microvessels from frozen human brain tissue. ( B ) Microscopic images of the human brain microvessel fraction stained with Trypan blue. Human brain microvessel fractions were obtained from four donors (Nos.1–4), as described in Table . Scale bar = 250 μm. ( C ) Total amount of protein in the brain microvessel fractions. ( D ) Amount of protein normalized to brain weight. Each bar represents the median of four independent experiments
Article Snippet: Frozen human brain tissue (frontal cortex) was obtained from ProteoGenex (Inglewood, CA, USA) of the KAC Corporation (Kyoto, Japan).
Techniques: Isolation, Staining